FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2521_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2521_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161108
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3130.19427961367529856No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCTAAG200.007327494751.785881
CCGAGCT200.007345519551.75372734
GTAAGCC200.007345519551.7537279
CAACGCC200.007354544551.73766367
CCGACTA459.9731405E-538.3360949
ATCGCCT400.002588824834.5024838
TCTAACC400.002592770834.4917762
GTATCAG605.379906E-428.7699341
TTAATAC605.4093346E-428.7431453
ATAGAAC700.001330725524.636983
AGAGTGT902.114311E-423.0016566
CAGGGGT750.001984802523.0016564
AATATAC750.00198839122.9945163
GACTTCA750.00198839122.99451657
TATTGAG953.0483297E-421.7910425
TAGAACT850.00410335220.295584
TTTATAC850.00411073920.289283
CATTTGG950.0078027618.15920340
ATATACT1150.00110406518.0012954
ATACTTT1200.001468620317.2512446
Produced by FastQC (version 0.11.2)