FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2516_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2516_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences62043
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1560.2514385184468836No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACACC150.002338364369.0493144
GTATAGT150.002345878268.993544
GATGTGC200.00730566851.78698319
GCAGCCT200.00730566851.7869839
CGTTGTC200.00737605751.66169459
TCCATGG306.243002E-446.0328758
TTGTTCC306.267952E-445.9956934
TGTTCCA553.2236046E-431.3606995
GAATGGA450.004609634630.6390461
ACTCCTT1851.0113855E-414.89346169
CTCCTTA1500.006510737413.77645269
GACTCCT2001.9625602E-413.77645268
CGACTCC2204.3948457E-412.52404767
ACGACTC2357.6550455E-411.72464166
TGCCGAC4401.3924E-710.95854169
CTGCCGA5351.4733814E-910.94391169
GACGACT2750.002841569310.019237565
GCTGCCG6009.73705E-99.7583268
CGCTGCC6503.5033736E-99.53754367
ACGCTGC6909.778887E-98.98464266
Produced by FastQC (version 0.11.2)