FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2514_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2514_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94371
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2090.2214663402952178No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAG252.5517697E-455.214441
TAGACAT200.007330607651.763545
GCAACCG200.007330607651.7635415
GTCTTTG200.007330607651.763541
CACCGAC200.007330607651.763547
AAGTCTG200.007376904651.68124456
TTGTACG400.002574273234.5273531
AGAGTAG400.002574273234.5273520
AGCGCAT450.00459551930.67468810
GTGTAAT450.00459551930.6746881
TCATACT500.007689696327.607224
GTGTAAG900.00568764519.171681
TATTATT900.00570513319.1615092
ATAATAT950.007770847518.1626421
TATTCTG950.007770847518.1626425
CTACTCT2201.6045124E-615.66098169
TCTACTC2756.459959E-815.03454269
AAACTGT1400.004129939214.76606855
ATCTACT3554.5474735E-1014.55809669
ACACTTA2102.9433446E-413.14629514
Produced by FastQC (version 0.11.2)