FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2512_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2512_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences75017
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1550.2066198328378901No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACTATC150.002348013768.991987
TATACTT352.2539385E-549.279995
ATTATAC400.002599379334.4499663
AGAGATG450.00458300630.68360133
ATGTATC450.004598031330.6631057
CATACGA605.3362077E-428.78511812
ATACGAA605.3362077E-428.78511813
GAGATGG500.007668845427.61524234
TATAAAC500.00774428327.5599753
ACATACG953.0037915E-421.8160911
TACGAAA800.002854133721.58883714
ACGAAAT1055.9023226E-419.73836515
GGTACAT1055.92902E-419.725178
GGGTACA1055.955819E-419.7119967
AGGGGTA900.005689348619.164445
TACATAC1250.00190224916.58022710
GATAGTG1350.003250999115.30088969
GTACATA1400.00406807714.79387959
AAGAGGA4101.8189894E-1114.2746169
GGATAGT1550.00813122713.3265868
Produced by FastQC (version 0.11.2)