FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2511_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2511_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences120031
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2540.2116120002332731No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGGT150.002346614169.0279623
CTGCCTG200.00733128551.7709739
GTGCGAA200.00733128551.77097347
AGATAAG200.007355525651.7277951
GTTATAC200.007355525651.7277951
TATTAGG404.9754926E-543.124482
CTGACTG400.002581965234.5139859
AGGATTC400.002581965234.51398518
TAGTAGA553.2400156E-431.3632562
CTATTAG450.004616048730.6535071
TGTACTG500.00769252227.6111875
TCGCACT750.00198282422.9997223
CGCACTC800.00287768921.571244
TAATCCT850.004089415520.3023414
TTTTATA1055.9804186E-419.7140462
TAGGACA900.00569177519.17443516
GCACTCC900.00569177519.1744355
GAGAGGT2556.548362E-1118.9172769
ACTCCTC1108.155822E-418.8258087
CACTCCT950.007776438318.1652556
Produced by FastQC (version 0.11.2)