FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2501_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2501_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77225
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1570.20330203949498218No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAGA200.00733399851.74854336
TAAGACA200.00733399851.7485434
GCTTTCC350.0013359439.4530333
GGGCTGT350.001340209939.4274642
ACGACAG350.001340209939.4274612
AAGACAG500.007692068327.599225
TCAAACC500.007692068327.599223
TAGGTGT809.492593E-525.85751269
ACTTTCT902.1018778E-422.9993528
AAGGCTT3050.020.34689568
TTAGGTG1055.9806596E-419.70096268
AGGCTTA3450.017.98783569
GAAGGCT3550.017.48113467
CTTAGGT1200.001466586117.23834267
CGAAGGC4050.015.3229766
ACGAAGG4700.014.6709365
GACGAAG5401.0913936E-1112.13068564
CGACGAA6007.4578566E-1110.91761763
CCGACGA6452.8376235E-1010.15592262
GCCGACG7051.4279067E-99.29158961
Produced by FastQC (version 0.11.2)