FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2499_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2499_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223153
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6410.28724686649966613No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGGGG150.002358547668.959423
ACAGGGA350.001340478239.45846623
GACTTCG400.002593644234.4951747
CCTGGAC501.8630539E-434.479713
GCTTCCG450.004592648230.69680828
GCCTCGC450.004592648230.69680826
TCTGTGT500.007718580327.6023338
TACACAG500.007727038627.5961425
GTTACTC500.007743976527.5837673
TATAGAG500.007743976527.5837672
GTATTAA658.6648506E-426.5228521
CGCACTC750.001976684423.02260827
TCTTACA750.001994830822.9864732
CAGTAAC750.001994830822.9864731
TCGCACT800.002875736721.58369326
ATAGAGC800.0029020821.5498183
AGTAACC800.0029020821.5498182
GCACTCC850.00408661220.31406428
TAGGACA1206.386435E-520.1131634
TGGGGTC900.00572485419.1639865
Produced by FastQC (version 0.11.2)