FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2498_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2498_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211841
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4510.2128955206971266No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGATC200.007349491651.75230411
GACCGGT200.007363235651.7278637
ATGTAGG450.00461325930.668031
TACGCAA500.00770143527.61427714
CATACGC500.007710325527.6077512
TTTATAC851.4443738E-424.3367773
TCTAGAC1052.2811118E-522.9847343
CAAACCT953.0679518E-421.7750114
ATAGACT800.002902876621.5481956
TCTACAC800.002902876621.548193
TGCGAGG1004.3036757E-420.71070721
ATAACAG850.004125100620.2806473
GCAGTGT2900.020.21071469
CTAGACT1206.388212E-520.111644
ACGCAAT900.005702228319.1765815
GCAATCG900.005702228319.1765817
GTGCGAG1108.177949E-418.82791520
GCGAGGT950.00776940818.17587922
TGGTGAT950.00782254918.15441310
GAGGTGT1150.001098831818.01782824
Produced by FastQC (version 0.11.2)