FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2497_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2497_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182688
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4450.24358469083902612No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGATAG400.002587108434.5095945
CTTACCT400.002587108434.5095944
CTATACT652.3065211E-531.8550074
TAGACCC450.004606342430.6751944
CGAGCAG450.004606342430.67519434
GGACCAG500.007707693627.60767441
GTCTAAT500.00773868227.5849951
CTATTGC500.00773868227.5849951
TTAGGCA851.434107E-424.3597122
CGCACTC1103.240573E-521.9606527
CATAGGC1055.9851795E-419.71976733
GTCCTAG900.00573434219.1562461
TCCTAGA1301.17904056E-418.5668222
TAGGACA1702.403176E-618.2697834
GCATAGG950.007797367418.16294332
TCGCACT1351.5644295E-417.89386226
GCACTCC1351.5644295E-417.89386228
ACCTCGC1552.2239545E-517.81140114
CTCTAGG1552.2397411E-517.796771
ACTGTTC1200.00146760817.2547958
Produced by FastQC (version 0.11.2)