FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2496_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2496_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences185743
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3780.20350699622596813No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTACA200.007346417351.75525321
TGGGCAG200.007346417351.7552538
ACTATGT350.001344231439.4325758
CGAGTCA450.00461052530.66977918
CGCCGTA450.004622695530.65326167
GAACGAG500.007714671527.60280231
TCATATC500.007714671527.6028022
ACTTTCC809.5129784E-525.8776268
TTAGGCA951.043769E-525.42363222
GAGTAGA700.001328853424.6453591
CTCTTAC750.001985596323.0023371
GCATAGG953.0501478E-421.79168326
GCATAAG800.002888678621.5646950
ATAATAC800.002888678621.564693
TATGACC800.002888678621.5646948
TTCATAG850.00410497220.2961793
TAGGCAT1206.345283E-520.12704323
GGCATAG1055.992833E-419.71628825
AGGCATA1301.1733533E-418.57880824
GTAGATC950.00780580318.15973752
Produced by FastQC (version 0.11.2)