FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2495_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2495_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170853
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2770.16212767700889066No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAGGGT150.002356464368.967794
TTAACGC200.007361943451.7258453
GACTAAC200.00737901251.69556867
GCGAGGT306.2563166E-446.0594525
AGGCCTA306.2835106E-446.0189532
GGAGTGC306.2925956E-446.0054711
CGAGGTG501.8407684E-434.54458626
AGGTGTG400.002573808234.54458628
GAGTGCG400.002588660234.50410512
ATAGGTT400.002603580234.4637163
TCGACCT652.3174305E-531.8312875
TTCGACC652.3174305E-531.8312874
GTGTCCC450.004589305330.69729629
AACGTTA450.00459589630.68829520
ACGTTAT450.00459589630.68829521
AGTGCGA450.00460249530.67930213
CAATGCG450.00461571530.661339
TGCGGAG605.3993624E-428.7534212
CGACCTC854.4152202E-628.3985026
CTACTAC500.007712302727.6032811
Produced by FastQC (version 0.11.2)