FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2491_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2491_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134326
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4030.3000163780653038No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTGCAC150.002351258968.998143
CCCCGAA150.00235473568.9724555
AGGGTTC306.292296E-445.9987566
AGCAGTG306.292296E-445.9987567
TAGTCGA350.00134129239.44219637
GTATTTG605.3744006E-428.7706531
ATAGGGG500.00771202527.5992553
CCACTTG500.00771202527.5992559
TCTAACC500.00771202527.5992553
TATAACA750.001988536422.9908182
GCGTATG953.0390368E-421.79700519
GATTTAT800.00287424521.5779911
AAGGGTT800.002886710721.5619185
TTGCGTA1055.9711444E-419.721117
ATTATCA900.005684968519.1804371
TGCGTAT1108.166086E-418.82468418
GATTGCG1301.16809024E-418.58334415
GAAGTGG950.007800617718.1574046
TGTACGA1756.29138E-515.77100310
CGTATGT1554.4753804E-415.58602920
Produced by FastQC (version 0.11.2)