FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2487_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2487_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63675
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG990.15547703180212014No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGACCC150.002352204268.9489067
TGACGTT200.0072804151.83392324
GATCGGG200.00730314751.7931141
ATTGAGC200.00730314751.7931140
AACCAGC200.00734877951.7116747
TATTGAA501.8498083E-434.4744532
GTTCTAG400.002587397134.474451
GTATTGA400.002587397134.474451
GAATAGA703.8139937E-529.5495281
ATGACCA500.00764976527.62299249
AAGGCTT1503.5006675E-416.0754468
TCACCAG1550.00803982413.34494910
GTCACCA1550.00803982413.3449499
TAGGCTT2355.1119256E-513.2029811
CCTCTAT1850.001673803313.07522420
CCGACGA3701.2478267E-813.03413969
GAAGGCT1850.001714041613.03413967
TTAGGCT2457.555454E-512.66408354
CTTAGGC2457.555454E-512.66408359
GCCGACG4154.5856723E-912.45085668
Produced by FastQC (version 0.11.2)