FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2484_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2484_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174387
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3240.18579366581224518No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCAC200.00734764651.751728
TACTTTG553.2451504E-431.364687
GTACTCA450.00461777530.6588884
TATATAG605.4116105E-428.7427082
TATAGCT605.4116105E-428.7427084
TTATAGC500.007726778727.5929993
AGACTAT658.6258375E-426.5393436
CGGTGTG658.640315E-426.5317275
GATATAC700.001329053824.6436771
TGTCGGT750.001989215622.9941652
TCGGTGT800.002893935721.557034
TATAAGA1004.3219005E-420.694752
GATTTGC850.004105597720.2947921
TTAATAT850.004112430420.2889694
TTAAACT850.004112430420.2889694
GTCGGTG850.004112430420.2889693
CAAAGAG850.004112430420.2889693
GTGTCGG900.0057142219.1673051
TATGACA1108.21249E-418.8134084
TTATACT1108.21249E-418.8134084
Produced by FastQC (version 0.11.2)