FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2480_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2480_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences91264
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1860.20380434782608695No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAGGTA200.00734091551.7438436
GTATAAT400.002585390334.495891
GTGATAT500.00770274727.5967129
CTCCACA500.00770274727.5967121
ATGAGGA500.00770274727.59671254
TAAAACA658.601877E-426.5353014
AATTGTG950.007786096518.1557315
TGCAGTG1200.001458520117.25740269
ATGCAGT1450.00515163814.28198768
CCCCTAG1450.005170437514.2741621
CCTAGTG1450.005170437514.2741623
TATGCAG1500.00644995513.80592167
CCCTAGT1500.006473448613.7983562
TAGTGAC1750.001121881313.7983565
CTAGTGA1800.001387659913.4150694
AGTGACC2000.003063335512.0735626
TAAGCCT2902.7496599E-511.8951354
AAGCCTA2802.630694E-411.0879655
AGCCTAA2954.2632667E-410.524176
TTAGGCT3451.6076163E-49.9988094
Produced by FastQC (version 0.11.2)