FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2478_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2478_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48798
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1050.2151727529816796No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGTT208.433446E-568.9989856
AAGCGTG150.002342145468.998987
TGTATAC252.5429897E-455.199183
CCCCTGG200.0072878251.802361
GAAGCGT200.00731749451.749236
TTGACTA200.00731749451.749239
GTGTATA450.00455720130.6976931
CCAGAGG1200.001449655517.2497443
CAGAGGT1200.001449655517.2497444
CTCCTTA1300.002475803715.9228469
ACTCCTT1600.00982492612.93730768
GACTCCT2050.00364017711.78031369
ACGACTC2701.8410046E-411.49982869
GACGACT2903.5716748E-410.706737568
CGACTCC2300.00857739210.49984468
CGACGAC3157.653405E-49.85699767
GCCGACG3600.00258914478.62487265
CCGACGA3650.00293385428.50672266
TGCCGAC3800.0042216928.17093164
CTGCCGA4100.0083519417.573057763
Produced by FastQC (version 0.11.2)