FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2477_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2477_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences77352
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1450.18745475230116868No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGTTAA150.002341369.043981
GTATAAA309.012012E-657.536651
CGGATGT200.00733352551.7495149
TAAGATA200.00733352551.749514
ATTTACC306.2751246E-445.9995723
CGTACTC306.2751246E-445.999573
CAGTTCT350.001340107539.42829
TTTACCC400.002581573634.49967624
TTGTACT400.002581573634.4996764
ATACTCT400.002581573634.4996766
TGTGACC400.002581573634.4996765
TATAGAA450.004596571430.666382
GGTTCCA450.004596571430.6663837
TTTATTC700.001322711924.6426263
TTATTCT851.4264772E-424.3527134
TACTCTG1356.68957E-620.44425269
TCTGTGT850.004086314720.29392869
CTCTGTG850.004086314720.29392868
CTACTCT2002.7785063E-818.97482369
TCTACTC2452.816705E-715.48965268
Produced by FastQC (version 0.11.2)