FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2476_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2476_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126064
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2050.16261581418961796No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTAGGT200.00733103951.7729458
GGGCGGT200.00733103951.77294519
CCATAAG200.00733103951.77294538
AGTCGTC306.276225E-446.02039321
ATTCTGC500.007692545727.6122386
GTATAAA658.6118426E-426.5396821
TATATAC809.518416E-525.865923
ATAGGCA900.00569208819.17516540
TGGTGAA900.00569208819.17516544
GGATAGT1451.2583534E-519.02025269
GGCATAA950.007776859718.16594543
ATAAGCA1351.5575795E-417.8968240
TAAGCAT1351.5575795E-417.8968241
GGCATAG1200.001462563217.25764725
GCATAAG1855.347154E-616.79122550
TACAATT1503.482273E-416.10713824
AAGCATA1300.002497613415.93013842
AGCATAA1300.002497613415.93013849
CCCCTAG5850.015.9238091
TTAGGCA1350.003212165315.34013222
Produced by FastQC (version 0.11.2)