FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_qg2475_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_qg2475_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81442
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1350.165762137472066No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAACGTC150.00234825368.99570564
CCCATTA252.5537823E-455.19656432
TTTAAGC200.007318617351.77857624
ATCTAGG200.00733643551.7467731
TCTGGCG200.00733643551.74677313
CCATTAA306.278712E-445.99713533
CATAGGC350.001340870639.42611333
GCATAGG400.00258303734.4978532
GGCATAG450.004599163330.66475731
AGGCATA809.4592826E-525.87338636
TAGGCAT750.001977965222.99856635
GAGAGGT953.0331782E-421.78811669
GAGAACA850.00408938220.2928544
CACACCT1450.005162130614.27497314
ATAGAGA2204.3603274E-412.54467367
GACGAAT3451.317703E-510.99931469
CGACGAA3551.808235E-510.68947568
TAAGCCT3301.02176666E-410.4538944
TTAGGCT3702.8364633E-510.26242222
GAATAGA2700.002421079710.22158468
Produced by FastQC (version 0.11.2)