FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_nic894_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_nic894_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9998802
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAG18900.014.9673581
TATACCG14750.014.7336785
GTATATA27600.014.4991321
GTATTAG16600.013.3004081
GTATAAT40550.013.1866321
TTATACT46050.013.1828334
CGAATAG260200.013.07377269
GTATAGG13950.012.8594461
AGAGGTG60850.012.80085569
CTATACT19050.012.3123124
GAATAGA259650.012.26520369
ACGAATA323900.012.25836769
TATAGTG18050.012.2310975
TCTATAC22700.012.1554283
GTATAAA40100.012.0441661
TACACCG13500.012.0095495
GAGAGGT76400.011.774384569
GTATTAA21700.011.7642721
TATACTA15600.011.719635
GTATAGA23650.011.6694921
Produced by FastQC (version 0.11.2)