FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_nic1192_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_nic1192_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14445683
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG156410.108274562026593No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATAGTG142250.030.62905769
GGATAGT218100.025.6350469
ATAGTGT78900.023.59150169
AGGATAG319900.022.80028369
GAGGATA459250.018.2012669
AGAGGAT642650.017.82893269
AAGAGGA940050.016.96997669
TAGTGTA39050.016.41839669
GAAGAGG1227300.016.40496469
CGAAGAG1531800.015.56069369
AGTGTAG31950.014.45681969
TACACCG18700.014.388395
TATACCG18750.014.3500195
TATACAG23700.014.1183045
GTCCTAG10700.013.8618531
GTACTAA17700.013.6414761
GTATTAT36800.013.4974361
ACGAAGA1915400.013.42871969
GTATTAA28700.013.3406711
GTATTAG18700.013.28091
Produced by FastQC (version 0.11.2)