FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_ju3170_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_ju3170_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6138152
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAT23900.015.8749261
GTATTAG13150.015.4754461
GTATATA19200.015.0901991
GTATATG14750.014.9659691
GTATTAA15350.014.6056841
ATAATAC26850.014.1294863
TATACTG18350.013.7226865
GTATAGA16750.013.5908321
GTATAAA32500.013.26610851
GTATAAT32700.013.184971
TTATACT32800.013.0389774
TAATACA27250.012.9100924
CTATACT12600.012.8653924
TCTATAC14750.012.6264053
GTATAAG11800.012.5690771
CGTATAC8600.012.0309733
TAATACT20700.011.996594
TTAATAC15850.011.9677193
TATTATA33050.011.8942492
CGATACT74550.011.78851469
Produced by FastQC (version 0.11.2)