FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_ju2572_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_ju2572_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5021600
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA11000.015.6807131
GTATTAT19300.015.0144851
TAGGCAT34000.014.21323829
GTATAAA24550.013.9114431
GGCATAG23550.013.77549631
AGGCATA35200.013.72787430
GCATAGG22400.013.71195232
GTATTAG11200.013.5526161
TATAATA24050.013.4771462
TATATAC16300.013.3294323
CATAGGC19550.013.23950733
GTATATA18250.013.0429161
CCCCTAG33550.012.9558691
TTAGGCA26150.012.9356922
GTATAGG6750.012.7768771
CCTAGTG32450.012.64709953
GTATAAT21700.012.4000471
TATTATA22200.012.2704152
CCCTAGT34350.012.0459112
TATAGTG11500.011.9964055
Produced by FastQC (version 0.11.2)