FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_ju2505_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_ju2505_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5590691
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGA15300.017.3600461
TTATACT28700.017.0655064
GTATTAT20000.015.0051671
CTTACGT23950.014.96843269
CCTTACG28700.014.5328969
GTATATA19950.014.5240581
GTATAAT26950.013.951451
GTATTAG10900.013.9244421
GTATAAG11350.013.6762891
GTATAGG9150.013.5716621
TATACTT22000.013.4839065
TATACTG18000.013.22258955
GTATTAA12050.013.1680781
GTATAAA25150.013.166941
TTTATAC23350.012.9987253
ATTATAC29050.012.8227923
TATTATA27600.012.7452812
TATACAG16050.012.6799315
CTCCTTA51550.012.63809569
TTATAGT16550.012.504494
Produced by FastQC (version 0.11.2)