FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n02_ju1975_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n02_ju1975_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12193356
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG20050.014.1074721
GTATAAG24800.014.048161
GTATATA45050.013.7824821
GTATTAG27200.013.6963411
TATACTG43900.013.2793655
GTATATG36100.013.0907081
CGATCTA135400.013.05969
TAGGACA32550.012.9276444
TTATACT54300.012.7675264
GTCCTAG25400.012.7656771
TATACCG20450.012.6509475
TCTATAC32250.012.6191173
TATACAG39150.012.1591275
GTATAGA35600.012.0149351
GTATAAA54050.011.8704441
TAATACT37550.011.8492354
GTATTAT44100.011.73282051
GTATAAT51850.011.7088341
CTATACT28600.011.5775244
GTATTAC30000.011.3831821
Produced by FastQC (version 0.11.2)