FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_8_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_8_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences303429
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTTAA252.554405E-455.242621
CCTTACA400.002594005534.49818442
TACATAG652.3167657E-531.8444795
GTCTTAC450.0045999830.6903441
TACACCC605.4145826E-428.748494
GTATAAG658.645006E-426.53706615
TAAGCCA700.001331994224.64156240
ATTATAT953.0561083E-421.791922
GTATTGG800.002881631521.5791471
GTGTATA850.004114560320.2930513
GCGTCAG850.004114560320.293054
TATAAGA850.004114560320.2930516
GTGCAGG900.00569941619.1814651
CTGTACC950.00782388418.1569424
TGTATAA950.00782388418.15694214
TGTAGTC950.00782388418.1569425
GTTTGAG1400.00410042714.7971311
CTAGGCA6400.014.55872169
TAAGGTG1500.006516515713.7992755
AACAGAT1550.008095277513.3541377
Produced by FastQC (version 0.11.2)