FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_7_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_7_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114562
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTACGT150.00235094968.993893
ATCTACG200.007332117851.76801359
ACGGGTC200.007344793551.745429
GTATGAT350.001340450739.4422951
CGTTACG350.00134333339.425082
TGATCGC450.00460751330.6639483
GATGTCG450.00460751330.66394851
TATTGAA450.00460751330.6639482
AGACCAA450.00460751330.6639486
AACAATG500.007709690427.5975557
GTGTGAG500.007709690427.59755511
TGAGGTT500.007709690427.59755510
CAGTGGA900.0057065419.1649689
CTCGCTT950.00779657418.15628622
CTTTCTG1351.5623811E-417.88730426
TCAGTTG1200.001466691617.2484728
CTAGGCA3859.822543E-1114.34265369
AGGCATG2557.3285737E-613.534121569
ACTAGGC4651.4551915E-1012.61729469
TAGGCTT2100.00442281611.4989815
Produced by FastQC (version 0.11.2)