FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_6_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_6_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144342
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCCGGGA200.00734767451.74714321
CCCCGGG400.002589550634.49809620
TAAACTG501.8539869E-434.4980935
AATCGGC450.004610691730.66497210
ATATAAA703.820374E-529.5800461
GGTGAAA500.007701927327.608041
TTACATG500.007714972427.5984753
ATAAACC700.001328633224.6414973
ACCCTGT1400.004109118614.78489858
GAGACTA8150.013.13107469
CGAGACT9050.011.82522268
TGATCTC2050.003682050411.78800769
CATGATC2100.004408342311.507339567
ATGATCT2150.005254019511.23972768
ACGAGAC10200.010.49198667
ACTAGGC4906.1675746E-79.86343469
AGGCATG3157.8027614E-49.86343469
CTAGGCA4201.0674001E-59.86343469
GACTAGG5654.113099E-89.77614769
GGCATGA2850.00379752489.69039168
Produced by FastQC (version 0.11.2)