FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_5_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_5_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29622
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTGTG150.00233288669.010
TGAGCAA150.00233288669.020
ACTCTGC200.00728878951.756
GTATCAA200.00728878951.751
GACTAGG1501.6050484E-518.469
AGACTAG1704.7040547E-516.23529468
GAGACTA1807.669575E-515.33333367
CGAGACT1901.2161593E-414.52631666
ACGAGAC2001.8809173E-413.865
CACGAGA2102.8442524E-413.14285764
CCACGAG2500.001233273811.0463
CCCACGA2700.002342564810.22222262
AGCCCAC2900.00423476479.517241560
GCCCACG2950.00487646139.35593261
GAGCCCA3450.00171606459.069
CGAGCCC3600.0025269848.62568
CCGAGCC4108.597371E-48.41463467
CTCCGAG4400.00172968717.840909565
TCCGAGC4400.00172968717.840909566
ATCTCCG4650.00297752147.419354463
Produced by FastQC (version 0.11.2)