FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_4_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_4_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences168565
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAGG150.002350728369.009792
GCAGCGT501.8466986E-434.5253871
GCGAAAG450.00461511730.6619242
CACACTG750.001984374423.0032655
AATACAA800.002886908121.5655615
ATGTAGA900.0056903119.1807691
CAGCGTC900.005709875419.1693862
AGGAGTG950.00780107618.1604715
GGCATGA3300.017.78580569
CTGCTGT1400.004115658314.78342651
TTCGAAG1450.00518559614.2778872
AGGCATG4202.7284841E-1113.97041368
GAACTGA1600.00994670912.9393376
TAGGCAT4802.5102054E-1012.22048467
ACTAGGC6401.8189894E-1211.32864369
CTAGGCA5401.7626007E-910.869102569
CCTTATC2300.00867752910.5077271
GACTAGG7600.010.44847169
GAGACTA10150.010.20454869
AGACTAG8659.094947E-129.17743568
Produced by FastQC (version 0.11.2)