FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_40_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_40_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences330901
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACACC450.00461867730.6657284
GTTCTAG756.151746E-527.5991541
CACTAGG500.00772824827.59915214
GTATAGC500.00772824827.5991523
ATAACAG1253.4916848E-622.0793235
TTAGGCA953.0598755E-421.78880740
ATTACAC953.0598755E-421.7888073
GTGTATA850.00411481520.29349513
TACACCA850.00411481520.2934955
TACAAAC1206.3710126E-520.1243843
TCTAGTG1056.011808E-419.7136823
TAGAGCG900.00572698719.166087
GTGCATA950.00782435618.157341
ATAGACA950.00782435618.157344
CTAATCT1150.001108181517.9994474
CTGGAGT1452.715705E-416.6546634
ACCGAGG7750.015.58723469
AGGCATA1554.5130588E-415.58016742
TAGGCAT1605.744044E-415.09328841
CCGTTCC1400.004123420514.785269
Produced by FastQC (version 0.11.2)