FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_3_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_3_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135708
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGAGTA200.007345286751.749725
TTAGGAT200.007345286751.749724
GCTTTAG200.007345286751.749721
ACCGGAC400.002588375734.49981723
GTATAAG400.002588375734.4998171
TCCTGGA553.242131E-431.363472
CAAGTCC500.00771149827.5998543
GTGGATT500.00771149827.59985456
TCACCAC850.0041019120.294013
ATGGGGC950.00780006918.15779713
TTCGGAG950.00780006918.1577972
GATCTCG1807.9770754E-515.33890269
GTGATGA1350.00322351715.3332519
TGGATAT1550.00806253813.35476831
CATGATC2457.626687E-512.67340266
ATGATCT2357.648992E-411.74461967
TGATCTC2357.648992E-411.74461968
GCATGAT2853.117329E-410.89467965
GGCATGA3005.006737E-410.34994564
ACTAGGC5351.7676939E-810.32150569
Produced by FastQC (version 0.11.2)