FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_39_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_39_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences418181
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAGCGT1651.1459633E-1025.1020891
GTGTATA1651.1459633E-1025.09008413
TAGCCTG700.001332684324.6420465
TGTATAA1803.4197E-1022.99924314
GTATAAG1906.712071E-1021.78875515
ACTAGAC800.002896935421.561793
GCGTCAG2201.891749E-1020.3856934
ATATTAC850.00410526520.303161
TGAGCGC850.004116655320.293458
TATAAGA2051.7316779E-920.19445816
TTAAACC900.00572953519.1660374
ATGTGTA2354.5656634E-1019.08447811
TAGAGGA1301.1790438E-418.5763114
AGCGTCA2255.506081E-918.3993933
TGTGTAT2255.506081E-918.39939312
GGTTAGG950.00780625718.1659851
TTAAAGA950.00782782418.1572954
CAGCGTC2307.21775E-918.001562
GTCAGAT2359.433279E-917.6164426
CGTCAGA2702.903107E-916.6105635
Produced by FastQC (version 0.11.2)