FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_38_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_38_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences373247
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTTC450.00461974330.6652978
CTTTAAG500.007730020727.598779
TAGACAG658.641587E-426.540835
CTCTTAA700.001329388424.6516651
GTATTAT750.00198638323.008221
TACACCG750.00199103322.9989747
GTACTGG1851.1650627E-820.5208451
TACCCTT850.004112925420.295935
TAGAATA950.00782077318.1595174
TAATATT1200.001473367717.2515414
TTTACCC1200.001473367717.2515413
CTAATCT1503.5147677E-416.1014394
GGCATAG1503.5183574E-416.09928143
ACCATGC1300.002518183315.9223658
ACCTGGA1554.50627E-415.5841252
GCGCAAA1554.515465E-415.57994911
AGCGCAA1808.065133E-515.33264810
ACCTTAA1808.065133E-515.33264819
TAGGCAT1605.7470973E-415.09307753
TTAATCC2101.8062663E-514.78505522
Produced by FastQC (version 0.11.2)