FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_37_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_37_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences432935
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATATGG400.002593809334.502022
TAACGCA450.004620827730.6649194
GTTCTAA906.8837817E-626.8380071
GTATAAG1306.2791514E-926.54001615
GTATTAC809.5370364E-525.8795051
GTCCTTA700.001331152924.6471481
TATGGAC851.4406984E-424.3543683
TAGGGGT851.4418285E-424.3515554
GTATAAT1151.6910071E-624.004181
GCAGCGT1301.8387618E-723.8887771
TGTATAA1452.0063453E-823.79449814
ATGTGTA1653.1232048E-923.00400511
TGTGTAT1502.8712748E-823.00400412
TCCTAAG750.00199034923.0013472
CTTACCC750.00199034923.0013473
TTATACT1202.453171E-622.9986924
TACACCG750.001991686922.9986925
TAGGCAG800.002895559921.56376353
GGGATAG1808.54925E-921.08213261
GTCCTAA1004.3237067E-420.7036081
Produced by FastQC (version 0.11.2)