FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_36_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_36_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences341009
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTATA1450.035.68866713
GTTATAG400.00258482234.5243571
CAGCGTC1450.030.9347132
CTTACAC450.00461549130.6703193
GTATAAG1600.030.18666515
GCAGCGT1501.8189894E-1229.921111
AGCGTCA1551.8189894E-1228.9389273
TATAAGA1700.028.4109816
TGTGTAT2150.027.27831612
GCGTCAG1653.6379788E-1227.1850514
TGTATAA1800.026.83259214
ATGTGTA2200.025.09021611
GTATGAA1001.5509722E-524.1670511
GTCATGG953.0449373E-421.804861
TATACAG800.002893298921.5650654
GTCAGAT2201.891749E-1020.3858016
GGGTTAC850.00409755120.3084471
CAACGCG850.004114994320.29355857
CCCGACG850.004114994320.29355812
AGATGTG2655.456968E-1219.5277639
Produced by FastQC (version 0.11.2)