FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_35_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_35_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295641
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTTA350.001346877239.424516
GCAATAG553.2531857E-431.36040738
TATATGG605.399779E-428.7616332
CCTTTAG605.4158474E-428.7470388
TTAAACT605.4158474E-428.7470384
GCCTGGT500.00772966227.59715811
CTTTACG500.00772966227.5971589
CCGTGAG658.6470234E-426.53572713
TAATTCC800.002896125721.5602784
ATAATGT850.004111483720.2954623
CTAATTC850.004111483720.2954623
GGCTGAG850.00411551420.2920287
TATTCCA1551.0002877E-620.0301955
GTATAAA1258.60725E-519.3507421
TATGCCG900.005716773719.17117957
AGCGTCA900.005722366719.1679343
GTACAAG1108.1295666E-418.8481251
GAATAAG1301.1629906E-418.6064821
GTATGCC950.00782569818.15602556
GAAGTTA950.00782569818.1560256
Produced by FastQC (version 0.11.2)