FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_34_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_34_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences433477
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTATTAA501.8520687E-434.5235441
GGGTAGC450.00461977530.6663596
CTTAGAC658.6488645E-426.5381973
ATTAAAC902.1235304E-422.999773
GCTTAGA750.001991147922.999772
CCATACT902.1235304E-422.999774
AGCGTCA1507.3111914E-720.6997933
GTATAAG1356.807806E-620.4442415
CTATATC850.004116348520.2939134
TCTTACC850.004116348520.2939132
TCCTACT850.004116348520.2939132
TATAAGA1409.322181E-619.71408816
GTGTACG900.005706231619.1797491
CTTACCG900.0057291119.1664733
TACGCAG1108.2259823E-418.8179935
GCGTCAG1651.8231804E-618.8179934
GTGTATA1702.4254114E-618.26452313
ACCATAC950.0078272418.1577133
TTAAACA950.0078272418.1577134
GTATAAA1402.0679945E-417.2617741
Produced by FastQC (version 0.11.2)