FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_32_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_32_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences400167
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGGCG450.00462015230.6652764
GTATATA605.40157E-428.7630771
GTACTAG500.007711860427.6125531
TAATACT500.007730701527.598754
GGTCGTC809.549507E-525.8738297
GTATAAG1001.5593925E-524.15192615
GCTTGTG800.002896927321.5615237
CATACAC800.002896927321.5615233
ATATGGC850.004116645520.2931983
CACAGGT850.004116645520.2931984
GTAGTCT850.004116645520.2931984
GTGTAGT1055.9951906E-419.7232511
GCCGGGA1056.0154183E-419.71339241
TGTAGTA1056.0154183E-419.7133922
CTAGACA900.00572952419.1657984
ATTGGGC900.00572952419.1657983
TACAGTG1301.1789944E-418.5760825
TACTTTA950.00782780718.1570727
ATAATAC950.00782780718.1570723
ATACTTT1903.4850382E-718.1570726
Produced by FastQC (version 0.11.2)