FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_31_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_31_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences334740
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT350.001346465239.42848613
TTGGGCC450.00461810830.6666014
TTAAACC500.007727297527.59994141
TACCCAG809.542131E-525.8749455
GTATAAG809.542131E-525.87494515
TTATACT1151.6916911E-623.9999494
GTAAGCT750.001990113922.9999524
GTATAAC1103.254298E-521.9577791
GTATGAG800.002892713321.5656761
TCATAAG1004.3260303E-420.6999573
GTTCATA850.004110673420.2971081
TGTATAA850.00411423220.29407514
GCGTCAG850.00411423220.2940754
TATAAGA1056.010852E-419.71424516
TTATGGG900.00572617819.1666263
GTTCTAC1108.2119426E-418.8209531
GTCTAGT950.00781651718.1605681
TGTAGTA950.00782325518.1578562
GTTATAC950.00782325518.1578563
ACGCTAC4600.017.9999669
Produced by FastQC (version 0.11.2)