FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_30_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_30_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences435670
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAGCG404.9942893E-543.1288035
CTAGTAA501.8573095E-434.5070041
GTATACG501.8573095E-434.5070041
TGTAGTA450.004617580730.6693732
TCAATAC854.4168246E-628.414273
ATCGTGC1005.006823E-727.59926634
CTTGTAC906.8885165E-626.8357013
TAATACT658.643976E-426.5408024
TCTATAT700.001331833524.645033
GGTAGGG1001.5610898E-524.14935728
TATACGG750.001990027323.0020282
CGTGCGC1052.277043E-522.99938836
TAGGCAT1103.261581E-521.95396241
TAATAGG953.057195E-421.7938981
GATAGAC800.00289702221.56192641
TCGGTAG1455.278762E-721.41322326
CGGTAGG1004.329632E-420.6994527
GGTGCCC850.004116776420.29357745
TCGTGCG1206.376746E-520.12446435
TACATGG1056.011187E-419.7160242
Produced by FastQC (version 0.11.2)