FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_2_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_2_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147159
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATATAGC200.007346357751.756
GTATCCT200.007346357751.7526
ATGTATT450.00460973830.6666661
TAGTTCC450.00460973830.6666664
CTTATTC500.0077133827.63
AGAGCTT756.127627E-527.5999985
ATAGAGA700.001328330524.6428594
GCATGAT1756.0917955E-921.68571569
TTGACAG800.00288756521.56255
TACAGAG800.00288756521.56255
GGCATGA1952.1163942E-819.46153868
AGGCATG2208.421921E-817.2567
GAAGTGT1250.001929797116.5600016
AGACTAG4900.015.48979669
AGAAGTG2001.9630481E-413.85
CATGATC1550.00806650213.35483869
GAGACTA5700.013.3157968
TAGGCAT2851.5832484E-613.3157966
CTAGGCA2901.924982E-613.08620765
ACTAGGC3256.8856334E-611.67692364
Produced by FastQC (version 0.11.2)