FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_29_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_29_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences160152
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTC200.00734766751.74976761
AGTGCGG350.001344380439.42839410
TAAATAC350.001344380439.4283943
CTCCGAC400.002589753134.49984436
GTTTCGT605.391927E-428.7588481
CTTCTAT500.007703799827.6084941
ATGTGTA500.007715554427.59987311
GCCAGAC700.001328878824.64274657
ACCCTTC750.00198563822.9998958
TCTACTG750.00198563822.9998953
CTATTAT850.00409765120.3003651
TTTGTAC1055.9921877E-419.7141953
GCTACGC2307.1286195E-917.9999269
AACTTTG1402.0685857E-417.2499227
CGCTACG2959.231371E-915.203320569
ACGCTAC3901.2369128E-1014.15378269
TAAGGTG1800.001394485613.4166065
GACGCTA4408.3491614E-1012.54539868
AGACGCT4802.5102054E-1012.21869567
AAGGTGA2450.001086455511.2652566
Produced by FastQC (version 0.11.2)