FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_28_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_28_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161022
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACTCA150.002352699968.9937933
CACTTAA252.561678E-455.195037
CTTTAGG200.007341196851.7614171
AGGGCGG400.002586934234.5076146
CCTCGTC652.3106515E-531.8432889
GGAACCA553.2464112E-431.3608155
TACTCTC500.007718892327.5975157
GTACGAA500.007718892327.59751557
CGCTACG2707.2759576E-1219.17685369
GCTATCT950.007796058418.161968
AATCTTC1200.00147021217.2484476
ACGCTAC3601.8189894E-1216.30032369
CGAGCAA1503.4957647E-416.10355236
GGGAATA1503.504053E-416.0985520
AGCGAGC1400.00411282114.78438434
TATCCAG1400.00411282114.78438425
GACGCTA4001.0913936E-1114.66573568
AAGCGAG1550.00807563913.35363733
GCGAGCA1600.00995942412.93633635
GCTACGC2000.003073073512.07766468