FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_27_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_27_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences173375
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGT150.002353257768.991924
CCCCGTG200.00735195451.74394243
GGATGTC350.001345430939.42395831
CTATCCT459.987403E-538.328854
TCTCTAC450.00461462330.663083
GTATTAT500.00769979727.6126981
GTATCCA500.00769979727.6126981
CGTATGC500.00772152127.5967755
CCTTCGA658.6187E-426.543018
CATAGGC658.633241E-426.53535545
GATGTCA850.00410909420.29174232
GCTACGC2701.4733814E-1017.8971269
ACAAGTG1452.7081356E-416.6532235
TATTATT1250.001929453116.562842
GCGAGCA1756.313219E-515.76958135
CGCTACG3201.6461854E-915.10069568
GGGAATA2001.968183E-413.79838520
AGCGAGC2001.968183E-413.79838534
CTACGCT1800.001390412313.4228469
ACGCTAC3701.2698365E-813.0600667
Produced by FastQC (version 0.11.2)