FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_26_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_26_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences328602
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATAA751.6651811E-632.19815414
AGTATTA450.004619233330.6649072
GCAGCGT854.3965956E-628.4274391
AGCGTCA854.417485E-628.4101353
CTCCTAA500.007706240827.6152251
GTATAAG906.889557E-626.83179515
GTGTATA1057.6593824E-726.28420613
TTACCTA851.4408006E-424.3515454
TATAAGA1103.2592085E-521.95328716
CAGCGTC1154.591827E-520.9987962
TGTGTAT1501.6887625E-518.39894512
GCGTCAG1351.5732979E-417.8878634
ATAGGCA1200.001474308317.2490152
ATGTGTA1402.0777913E-417.2490111
ATTGTGC1402.0777913E-417.249018
CATAGGC1602.941436E-517.2490151
TAAGCCA1402.0777913E-417.2490146
GTTATGG1200.001474308317.249013
GGCATAG1653.8300335E-516.72631549
ACGCTAT2702.8794602E-916.61774469
Produced by FastQC (version 0.11.2)