FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_24_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_24_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences246839
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATATA350.001346191539.4260254
CCTGGAC652.315216E-531.8440973
GTACTAA450.0045989130.6895581
ACTACCC450.00461719230.6646883
AAGGACT450.00461719230.66468826
GTTACTC500.007725783627.5982173
CGGGCGT500.007725783627.59821727
CTTATCG700.001331383724.64126629
TCTGAGC850.004112705620.2928073
CTGAGCT850.004112705620.2928074
TGCTCGG850.004112705620.29280710
TACTCAC900.005724070619.165435
CTAATCT900.005724070619.165434
ATTACAT950.007820418.1567213
AACTATT950.007820418.1567212
AACCGAT1150.001107348217.9988387
CTGAATG1804.1718504E-617.2488869
GTCACCT1452.712897E-416.65409735
TTCTGAG1300.002515276215.9220492
ACCTTAA1808.048843E-515.33234419
Produced by FastQC (version 0.11.2)