FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_23_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_23_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences238816
Sequences flagged as poor quality0
Sequence length75
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCGA350.001346058339.42629610
AGGCTAA400.002592968534.49801348
TTAGGCG450.004616738830.66489834
TAGGCGT450.004616738830.66489835
TTAATGT500.007725028327.598414
ACACTGC756.145815E-527.5984088
GGTTTAT800.002890236421.5657711
ATTGTAC800.00289375221.5612563
TTCGCGA800.00289375221.56125617
GTTGGGG850.004112156620.2929463
TCAGTAC850.004112156620.2929463
TAGAACT1056.0061767E-419.713154
ACTGGTC1108.2138396E-418.8170978
GTCAGAT1150.001107154317.9989622
CGTCAGA1402.0714816E-417.2526171
GATGTGT1250.001935298316.5590466
TTCAGTA1503.5120736E-416.0990722
GAACAAT1300.002514839615.9221576
GGATTAA1350.003229738215.335661
ACCAGAG6550.014.22949369
Produced by FastQC (version 0.11.2)