FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_22_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_22_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences210170
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATAG306.300594E-445.9985735
GATTACG400.002588900334.507141
TCCCCAC400.002591924534.4989315
GGGTAAG450.004609517330.6730141
GTATGTC450.00461488830.66571846
ATATAAT500.007712984527.6057111
GCAGCGT500.007712984527.6057111
TGCCCAT500.0077219527.5991445
GTATAAG500.0077219527.59914415
TATAACT500.0077219527.5991444
CCTCAAT700.00132862124.6479571
GTATAAA750.001985247723.0047591
AAGAGCG750.00198799822.9992875
ACTACTG750.00198799822.9992875
CTTTGAC750.00198799822.9992879
TGAGATA800.002892161321.56183214
TAATGGG3300.020.90844216
GAATAAT1004.3125587E-420.7042851
GGGAATA3700.020.51287520
GATGTGT850.004109906520.29348810
Produced by FastQC (version 0.11.2)