FastQCFastQC Report
Mon 27 Feb 2017
H55C2BGX2_n01_sp29_21_2017.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH55C2BGX2_n01_sp29_21_2017.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences131181
Sequences flagged as poor quality0
Sequence length75
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAAGAC200.007346029451.7474253
ACGCGTC200.007346029451.74742511
CTGTAAG450.00459187130.688541
GTTTTAA450.00459187130.688541
TATATTG450.0046090530.665145
AAGGGTT500.007712241327.5986275
CCACCGC500.007712241327.5986276
CACTGAA850.00410224220.2931082
TACTGCA850.00410224220.2931082
TCCGGAA900.005709582519.16571227
GAATGAT950.00780070318.15699233
AGAGAGG2402.2548193E-715.81774468
GAGAGGA2353.1544296E-614.68571969
GTTGATC1550.00802241313.3643651
CAGAGAG3153.359055E-713.14721667
CCAGAGA3256.844237E-611.68079566
ACCAGAG3501.5587893E-510.84645365
AAGCCTA2550.001514993610.82299117
AGCCTAA2600.001781158310.61485718
CTAAGCC2900.00440453379.5167683
Produced by FastQC (version 0.11.2)